Microplate system (Core)

(24 channels/80uL)

Systems Microplate System SETUP MICROPLATE MULTIPLE SETUP EXPANDED Microplate Multiple X3 Microplate Reader Set Microplate Data Setup Microplate Diagonal Cut 3D Zoom In Microplate Glass Plate Spots PCR Film On Microplate
Systems Microplate System
SETUP MICROPLATE MULTIPLE SETUP EXPANDED
Microplate Multiple X3
Microplate Reader Set
Microplate Data Setup
Microplate Diagonal Cut 3D Zoom In
Microplate Glass Plate Spots
PCR Film On Microplate

The microplate respirometry system is an easy to use, high-throughput system for measuring individual respiration rates in tiny aquatic and terrestrial organisms – even over a wide range of temperatures (5 – 40 °C). The entire system is easily transported, e.g., to remote field stations, when packed inside the included transportation hardcase.

 

FEATURES

  • Measure oxygen consumption or production rates in water or air
  • Determine respiration rates in tiny organisms using glass 24-well plates
  • Real-time O2 measurements inside each gas-tight well (closed respirometry)
  • Connect up to 10 x 24-well plate readers to a single PC via USB for high throughput
  • Available well volumes: 80, 200, 500, 940, and 1700 μl
  • User-friendly MicroResp™ software for Windows 11 for data logging, statistics, and analysis
  • Optical oxygen sensors can be re-used and re-calibrated for multiple trials
  • Non-invasive & non-destructive measurements
  • Re-usable borosilicate glass well plates
  • Compact design for use in incubators and/or on shaker tables

Study small scale metabolism

The microplate system is suitable for studying metabolic rates in small aquatic and terrestrial species, including model organisms like:

  • Daphnia and other freshwater and marine copepods
  • Zebrafish (Danio rerio) embryos and larvae
  • Medaka (Oryzias latipes) embryos and larvae
  • Killifish embryos and larvae
  • Xenopus sp. eggs and tadpoles
  • Fruit flies (Drosophila melanogaster etc.) and more!

Peer-reviewed papers using the microplate system

Here is a short selection of published papers referencing the microplate respirometry system:

  1. Effects of temperature on metabolic rate during metamorphosis in the alfalfa leafcutting bee
    Kayla N. Earls, Jacob B. Campbell, Joseph P. Rinehart, Kendra J. Greenlee (2023)
    Biology Open

    Link to article

  2. Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares)
    Rachael M. Heuer, Yadong Wang, Christina Pasparakis, Wenlong Zhang, Vernon Scholey, Daniel Margulies, Martin Grosell (2023)
    Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

    Link to article

  3. Chemical manipulation of mitochondrial function affects metabolism of red carotenoids in a marine copepod (Tigriopus californicus)
    Matthew J. Powers, James A. Baty, Alexis M. Dinga, James H. Mao, Geoffrey E. Hill (2022)
    Journal of Experimental Biology

    Link to article

  4. Metabolic responses to crude oil during early life stages reveal critical developmental windows in the zebrafish (Danio rerio)
    Karem N. Vazquez Roman, Warren W. Burggren (2022)
    Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

    Link to article

  5. Effects of marine mine tailing exposure on the development, growth, and lipid accumulation in Calanus finmarchicus
    Linn H. Svendheim, Tjalling Jager, Pål A. Olsvik, Ida Beathe Øverjordet, Tomasz M. Ciesielski, Trond Nordtug, Torstein Kristensen, Bjørn Henrik Hansen, Bjarne Kvæstad, Dag Altin, Julia Farkas (2021)
    Chemosphere

    Link to article

  6. Water-soluble fraction of crude oil affects variability and has transgenerational effects in Daphnia magna
    Mikko Nikinmaa, Emilie Suominen, Katja Anttila (2019)
    Aquatic Toxicology

    Link to article

  7. Genetic Variation in Metabolic Rate and Correlations with Other Energy Budget Components and Life History in Daphnia magna
    Sigurd Einum, Erlend I. F. Fossen, Victor Parry & Christophe Pélabon (2019)
    Evolutionary Biology

    Link to article

Other applications include examining mosquitoes, ticks, bedbugs, and other biological vectors, but also spiders, egg sacs, arthropod pupae, and even algae, bacteria and cell suspensions or isolated tissue and organs. In fact, you can measure the oxygen consumption (or oxygen production) of mostly anything that fits inside the glass plate wells.

Multiple well volumes

A core microplate system includes a single borosilicate glass well plate with either of the following well sizes:

Volume

(µl)

Inner diameter

(mm)

Depth

(mm)

80

4.5

5.0

200

6.0

7.0

500

8.0

10.0

940

10.0

12.0

1700

12.0

15.0

Any of the five plates is compatible with the same microplate reader included in the system. This allows you to run different sized well plates from the same microplate setup, thus making it easier for you to study multiple sizes and species of animals.

Automate your system with MicroResp™ software

The MicroResp™ software for Windows 11 is included in the core system. MicroResp™ is user-friendly software that can be used for data logging, treatment setup, sensor calibration, and data analysis. Using MicroResp™ will get the most out of your microplate respirometry system.

Want to try MicroResp™?

Downloading MicroResp™ from our website lets you run the software in demo mode simulating virtual hardware. Experience the intuitive user interface or use the analysis menu to explore real microplate data files from any PC with MicroResp™ installed.

Real microplate data example using Drosophila littoralis in a 200 µl well plate setup. Data from Professor Jesper Givskov Sørensen, Aarhus University.

A simple setup

One or multiple organisms are placed inside each of the gas-tight 80-1700 µl glass wells, typically using a pipette, spatula, or aspirator tube (when dealing with fleeing arthropods).

Seal the wells using a gas impermeable, transparent, and self-adhesive polyester film that sticks onto the surface of the glass plate. The glass material provides non-permeable surfaces; unlike ordinary well plates that are made of plastic materials that can act as a sink or a source for oxygen depending on sample pO2.

The sealed glass plate is placed onto the reader using the guide so that the 24 oxygen sensor spots are aligned with the 24 blue LED light sources and photo receptors. The reader, guide, and plate fits easily inside an incubator, or just in a climate-controlled room, to control the temperature of the glass plate.

An optional acrylic flow-through water bath provides a simple solution for temperature control. Place the water bath onto the reader, and the sealed glass plate inside the water bath, and then run thermostated water through the bath covering the glass plate. In addition, use the water bath for calibration with air and nitrogen.

For immobile organisms in water, like embryos, polyps, or sea anemones, use the orbital shaker table to minimize the risk of oxygen stratification inside the well space.

Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot for dynamic fluorescence quenching oxygen sensing. The plate is quickly cleaned using a mild soap and demi water or sterilized using ethanol or mild bleach ready for use in your next project.

Compact design, easy transportation

The microplate system takes up minimal lab space when in use, and requires only a single wall outlet for power, and it connects to your Windows PC via USB. The entire system is securely transported to even remote locations using the included hardcase.

Learn about how a team of researchers brought the microplate system with them to the Antarctic to study metabolism in terrestrial midge larvae:

Need higher throughput?

The core microplate system includes a single 24-well plate and reader. Should you need a higher throughput, simply expand the system with up to nine add-on systems each including one extra reader and glass well plate, i.e., for measuring respiration in up to 240 organisms simultaneously.

Need help?

If you need help setting up the microplate respirometry system, getting started with the MicroResp™ software, or if you need inspiration for your next project, have a look in large collection of quick guides and user manuals, our YouTube channel for useful video tutorials, and our ever-expanding list of peer-reviewed published papers using the microplate system.

Data

This is product data for the selected system:

Included

These products are included in the system:

Software

1
MicroResp™ v1 automated microplate respirometry software
MICRORESP PRODUCT (1)

Features

  • Individual sensor spot calibration (2-points)
  • Faster sampling rate (<5 sec)
  • Randomize wells (control/treatment)
  • Graph/analyse data based on treatments
  • Calculate respiration rates in real-time
  • Compensate for background respiration
  • Normalize oxygen values
  • Operate multiple readers from a single PC
  • Save data, graphs and statistics to Excel file

 

MicroResp™ is user-friendly software for Windows that will automate microplate respirometry, analyze respiration data, and get the most out of your oxygen microplate reader.

It replaces old and current programs entirely as this one piece of software handles device communication, calibration, data acquisition, analysis, and statistics.

Hence, this software will save you a lot of time for experiments and post-analysis, and provide more accurate oxygen and MO2 data with a higher time resolution.

Instrument

1
Microplate reader set (24 channels)
Microplate Reader Set

Features

  • Real-time oxygen measurements in 24 wells
  • Non-invasive & non-destructive measurements
  • Compact design for use in incubators or on shakers
  • Optional extension for monitoring of up to 240 samples 
  • 1- and 2-points sensor calibration by user
  • Sensors cleaned with chlorine or ethanol for repeated measurements

 

Use this 24-channel optical fluorescence oxygen system for high through-put (closed) microplate respirometry, or for monitoring oxygen conditions in (open) disposable plastic well plates. Using this reader with our re-usable 24-well glass microplates, oxygen consumption rates of tiny organisms can be measured in real-time inside 80-1700 µl closed gas-tight wells fitted with non-invasive and re-usable oxygen sensor spots. Up to 9 extra, and less costly, readers can be daisy-chained to the reader and splitter box included in this system, e.g., for real-time measurements of oxygen in up to 240 wells from a single PC.

Please consider optional MicroResp™ software for enhanced performance of the reader and quality of oxygen data.

Accessories

1
24-well glass microplate (80 uL)
GLASS PLATE 80 SPOTS

We have developed and manufactured this re-usable 24-well glass microtiter plate for high through-put respiration measurements in tiny aquatic organisms like zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers sealed with an impermeable PCR film for real-time oxygen consumption rate measurements. The glass material provides non-permeable surfaces, unlike ordinary well plates that are made of gas permeable plastics. Each well is fitted with an oxygen sensor spot allowing for non-invasive optical oxygen measurements when placing the microplate on a special reader. The glass microplate and sensor spots can be cleaned with chlorine, ethanol etc. between trials and re-calibrated and thus used again and again.

1
Microplate sealing film (100 pcs)
PCR Film On Microplate

Features

  • Adhesive clear polyester film
  • Waterproof film – prevents spills and contamination
  • Reduces sample evaporation

 

Self-sticking waterproof PCR film used for sealing our glass microplates for microplate respirometry.

Watch this tutorial on how to attach the PCR film.

1
Guide for microplate
Guide For Microplate

This microplate guide fits snug over the microplate reader offering a quick and effortless way of holding the microplate in place so that the 24 oxygen sensor spots are centered directly over the 24 LED light sources for optimum signal strength. The guide is made for applications where an incubator is used for temperature control of the microplate respirometry system. This guide is not needed if using the flow-through water bath for temperature control.

Optional

These products are optional for the selected system:

Systems

Systems Microplate System (Add On)
From 5,445.00 (EUR)
Microplate system (Add-on)

(24 channels/80uL)

Accessories

Water Bath For Microplate
Water bath for microplate

Bio Shaker Empty
Multi Bio 3D

Programmable shaker

PLATE ROLLER ISO
Plate roller for microplate sealing film

Compression Block
Compression block for microplate

Silicone Pad
Silicone pad

Files

These files can help you with the selected system:

Microplate

Title
Description
Version
Get
Title
MicroResp™
Description
A step by step guide on how to set up and use the software
Version
1.7
Get
Title
Microplate reader
Description
A data sheet for the microplate reader
Version
1.0.1
Get
Title
MicroResp™
Description
MicroResp™ software for Windows 11/10
Version
1.1.0
Get