MEASUREMENT SOLUTIONS FOR BIO SCIENCES

This miniature impeller pump is highly efficient despite the compact and lightweight design allowing for installation into the smallest places. With acetal impeller and inner parts, viton seals, stainless fittings and a solid construction it is made for continous use in hostile environments like marine labs.

Pump flow rate can be adjusted by varying input voltages between 6 VDC and 12 VDC. Further fine tuning can be made by restricting the outlet using flexible tubing and an adjustable clamp or by simply changing tube inner dimensions and thus friction against flow. The pump is quite happy to run even with the outlet completely blocked. Pressure inside the pump will increase; yet load on the drive motor will reduce as flow is reduced.

The pump mounts onto any planar surface with stainless screws and o-rings (both included). We can supply an optional fitting flange with barb hose fittings for 3.2 mm tubing.

The pump is NOT for submerged use.

We offer this clear and non-toxic silicone adhesive for mounting sensors spots on many substrates such as glass and most common engineering plastics. Suitable solvents include isopropyl alcohol, acetone or methyl ethyl ketone.

On exposure to moisture, the freshly applied material will "skinover" in ~7 min. Any tooling should be completed before this skin forms. After skin formation, cure continues inward from the surface. In 24 hours (at room temperature and 50 % relative humidity) the silicone glue will cure to a depth of about 3 mm.

For instructions on how to mount a sensor spot, please go to our FAQ.

This system measures individual respiration in real-time in tiny organisms like Zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers on a 24-well microplate footprint for real-time oxygen consumption rate measurements. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot that can be re-calibrated and sterilized for multiple trials. The microplate and reader is placed inside an incubator for temperature control, or use an optional flow-through water bath and refrigerated circulator. A soft silicone gasket with a thin liner (e.g. parafilm or PCR film) is placed on top of the microplate to seal the 24 chambers in one go.

Higher throughput?

Upgrade this system with up to nine add-on systems each including an extra reader and plate, i.e., for measuring respiration in 240 organisms simultaneously.