This system measures individual respiration in real-time in tiny organisms like Zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organisms are placed inside gas-tight 80-1700 µl glass chambers on a 24-well microplate footprint for real-time oxygen consumption rate measurements. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot that can be re-calibrated and sterilized for multiple trials. The microplate and reader is placed inside an incubator for temperature control, or use an optional flow-through water bath and refrigerated circulator. A soft silicone gasket with a thin liner (e.g. parafilm or PCR film) is placed on top of the microplate to seal the 24 chambers in one go.

Higher throughput?

Upgrade this system with up to nine add-on systems each including an extra reader and plate, i.e., for measuring respiration in 240 organisms simultaneously.

This add-on system will upgrade your (complete) microplate system with an additional 24. It includes everything needed for microplate respiration measurements including an extra reader that is simply daisy-chained to the first reader. Add up to nine extra readers in this way for simultaneous measurements in up to 240 chambers/wells from a single PC.